Fig. 5
From: Etoposide damages female germ cells in the developing ovary
Effect of etoposide on different stages of follicle development. Number and health of primordial (PF), transitional (TRNS) and primary (PRIM) follicles was determined in cultured mouse ovaries exposed to etoposide prior to (fetal ovaries: Ai, Bi); or after (neonatal ovaries: Aii, Bii) follicle formation. Figure shows: follicle distribution (Ai, Aii); and the percentage of follicles assessed as unhealthy for each follicle stage (Bi, Bii). c Photomicrographs of haemotoxylin and eosin stained cultured fetal and neonatal ovary sections, comparing healthy and unhealthy primordial, transitional and primary follicles. Bars denote mean ± SEM; n = 6 for all groups. Stars denote significant differences relative to control (*p < 0.05, **p < 0.01, ***p < 0.001)